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rabbit anti tsc1 primary antibody solution  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti tsc1 primary antibody solution
    Rabbit Anti Tsc1 Primary Antibody Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+tsc1+primary+antibody+solution/Hamartin%2FTSC1+Rabbit+mAb/bio_rxiv__2021__01__18__426261-152-33-58
    Average 95 stars, based on 133 article reviews
    rabbit anti tsc1 primary antibody solution - by Bioz Stars, 2026-10
    95/100 stars

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    Membrane:

    Article Title: Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex
    Article Snippet: .. The membranes were washed again in TBST buffer for 5 minutes three times before incubating overnight for 17 hours in their respective primary antibody solutions: the first membrane was incubated in 10 mL rabbit anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TSC1 antibody (Cell Signaling Technology, Clone #D43E2, Lot #3)], the second membrane was incubated in 10 mL rabbit anti-TSC2 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v rabbit anti-TSC2 antibody (Cell Signaling Technology, Clone #D93F12, Lot #6)] and the last membrane was incubated in 10 mL rabbit anti-TBC1D7 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TBC1D7 antibody (Cell Signaling Technology, Clone #D8K1Y, Lot #3)]. .. The membranes were then washed in TBST buffer for 5 minutes three times then incubated for 1 hour at room temperature in their respective secondary antibody solutions: the membranes blotted with rabbit anti-TSC1 and rabbit anti-TSC2 primary antibodies were incubated in 10 mL goat anti-rabbit secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit secondary antibody (Cell Signaling Technology, Product #7076S, Lot #33)] and the membrane blotted with rabbit anti-TBC1D7 primary antibody was incubated in 10 mL goat anti-rabbit F c secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit F c secondary antibody (Abcam, Lot #GR3347004-5)].

    Article Title: Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex
    Article Snippet: .. The membranes were washed again in TBST buffer for 5 minutes three times before incubating overnight for 17 hours in their respective primary antibody solutions: the first membrane was incubated in 10 mL rabbit anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TSC1 antibody (Cell Signaling Technology, Clone #D43E2, Lot #3)], the second membrane was incubated in 10 mL mouse anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v mouse anti-TSC2 antibody (Invitrogen, Ref #37-0400, Lot #UD288308)], the third membrane was incubated in 10 mL rabbit anti-TSC2 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v rabbit anti-TSC2 antibody (Cell Signaling Technology, Clone #D93F12, Lot #6)] and the fourth membrane was incubated in 10 mL rabbit anti-TBC1D7 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TBC1D7 antibody (Cell Signaling Technology, Clone #D8K1Y, Lot #3)]. .. The membranes were then washed in TBST buffer for 5 minutes three times then incubated for 1 hour at room temperature in their respective secondary antibody solutions: the membranes blotted with rabbit anti-TSC1 and rabbit anti-TSC2 primary antibodies were incubated in 10 mL goat anti-rabbit secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit secondary antibody (Cell Signaling Technology, Product #7074P2, Lot #28)], the membrane blotted with mouse anti-TSC1 primary antibody was incubated in 10 mL goat anti-mouse secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-mouse secondary antibody (Cell Signaling Technology, Product #7076S, Lot #33)] and the membrane blotted with anti-TBC1D7 primary antibody was incubated in 10 mL goat anti-rabbit F c secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit F c secondary antibody (Abcam, Lot #GR3347004-5)].

    Incubation:

    Article Title: Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex
    Article Snippet: .. The membranes were washed again in TBST buffer for 5 minutes three times before incubating overnight for 17 hours in their respective primary antibody solutions: the first membrane was incubated in 10 mL rabbit anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TSC1 antibody (Cell Signaling Technology, Clone #D43E2, Lot #3)], the second membrane was incubated in 10 mL rabbit anti-TSC2 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v rabbit anti-TSC2 antibody (Cell Signaling Technology, Clone #D93F12, Lot #6)] and the last membrane was incubated in 10 mL rabbit anti-TBC1D7 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TBC1D7 antibody (Cell Signaling Technology, Clone #D8K1Y, Lot #3)]. .. The membranes were then washed in TBST buffer for 5 minutes three times then incubated for 1 hour at room temperature in their respective secondary antibody solutions: the membranes blotted with rabbit anti-TSC1 and rabbit anti-TSC2 primary antibodies were incubated in 10 mL goat anti-rabbit secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit secondary antibody (Cell Signaling Technology, Product #7076S, Lot #33)] and the membrane blotted with rabbit anti-TBC1D7 primary antibody was incubated in 10 mL goat anti-rabbit F c secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit F c secondary antibody (Abcam, Lot #GR3347004-5)].

    Article Title: Polymeric assembly of endogenous Tuberous Sclerosis Protein Complex
    Article Snippet: .. The membranes were washed again in TBST buffer for 5 minutes three times before incubating overnight for 17 hours in their respective primary antibody solutions: the first membrane was incubated in 10 mL rabbit anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TSC1 antibody (Cell Signaling Technology, Clone #D43E2, Lot #3)], the second membrane was incubated in 10 mL mouse anti-TSC1 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v mouse anti-TSC2 antibody (Invitrogen, Ref #37-0400, Lot #UD288308)], the third membrane was incubated in 10 mL rabbit anti-TSC2 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v rabbit anti-TSC2 antibody (Cell Signaling Technology, Clone #D93F12, Lot #6)] and the fourth membrane was incubated in 10 mL rabbit anti-TBC1D7 primary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:1000 v/v rabbit anti-TBC1D7 antibody (Cell Signaling Technology, Clone #D8K1Y, Lot #3)]. .. The membranes were then washed in TBST buffer for 5 minutes three times then incubated for 1 hour at room temperature in their respective secondary antibody solutions: the membranes blotted with rabbit anti-TSC1 and rabbit anti-TSC2 primary antibodies were incubated in 10 mL goat anti-rabbit secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit secondary antibody (Cell Signaling Technology, Product #7074P2, Lot #28)], the membrane blotted with mouse anti-TSC1 primary antibody was incubated in 10 mL goat anti-mouse secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-mouse secondary antibody (Cell Signaling Technology, Product #7076S, Lot #33)] and the membrane blotted with anti-TBC1D7 primary antibody was incubated in 10 mL goat anti-rabbit F c secondary antibody solution [50 mM Tris, 100 mM NaCl, 1% v/v Tween 20, 5% w/v skim milk powder, 1:5000 v/v goat anti-rabbit F c secondary antibody (Abcam, Lot #GR3347004-5)].



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    Cell Signaling Technology Inc rabbit anti tsc1 primary antibody solution
    Rabbit Anti Tsc1 Primary Antibody Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+tsc1+primary+antibody+solution/Hamartin%2FTSC1+Rabbit+mAb/bio_rxiv__2021__01__18__426261-152-33-58
    Average 95 stars, based on 1 article reviews
    rabbit anti tsc1 primary antibody solution - by Bioz Stars, 2026-10
    95/100 stars
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